We are using cryo-EM to study the interfaces between diverse actin-binding proteins and actin filaments. Our studies support the concept that the actin structural landscape features conformations which are selectively recognized and / or induced by binding partners. This landscape could also be regulated by additional inputs (e.g. mechanical force or actin nucleotide state), providing a framework for establishing the local composition and architecture of subcellular cytoskeletal networks. Areas of interest include unconventional myosin motor proteins and cellular adhesion factors. We are also developing machine-learning based approaches for visualizing crosslinking proteins bridging filaments.